How to prepare single cell suspension of solid tisse?
Answers
Mass cytometry is a single cell biology technique that samples >500 cells per second, measures >35 features per cell, and is sensitive across a dynamic range of >104 relative intensity units per feature. This combination of technical assets has powered a series of recent cytomic studies where investigators used mass cytometry to measure protein and phospho-protein expression in millions of cells, characterize rare cell types in healthy and diseased tissues, and reveal novel, unexpected cells. However, these advances largely occurred in studies of blood, lymphoid tissues, and bone marrow, since the cells in these tissues are readily obtained in single cell suspensions. This protocol establishes a primer for single cell analysis of solid tumors and tissues and has been tested with mass cytometry. The cells obtained from this protocol can be fixed for study, cryopreserved for long term storage, or perturbed ex vivo to dissect responses to stimuli and inhibitors.