How to prepare stained temporary mount of onion peel?
Answers
Answer:
To Prepare Stained Temporary Mount of Onion Peel
Take a few drops of safranin solution in a dropper and transfer this into another watch glass. Using a brush, transfer the peel into the watch glass containing the safranin solution. Let this remain in the Safranin solution for 30 seconds, so that the peel is stained.
Answer:
Here is your answer
To prepare stained temporary mount of onion peel:-
Procedure:-
1. Tear a fleshy leaf of onion & collect a piece of the peel.
2. Spread the peel carefully on a slide.
3. Put a few drops of Janus green B stain on the peel. Allow it to stain for 5-10 min.
4. Wash the extra stain with the help of distilled water. Be careful so that entire stain doesn't get washed off.
5. Mount the cells in a drop of distilled water.
6. Use coverslip to cover the peel. Observe the slide under high power of a compound microscope.
The cells can alternatively to be mounted in the stain itself. A few air bubbles remaining inside the cover glass give a background stain that makes the viewing easy. (Note:-Adjust the microscope lens in sequence from Low power to high power.)
hope it's helpful