Biology, asked by Anonymous, 5 months ago

How to prepare stained temporary mount of onion peel?

Answers

Answered by ssinbox007
1

Answer:

To Prepare Stained Temporary Mount of Onion Peel

Take a few drops of safranin solution in a dropper and transfer this into another watch glass. Using a brush, transfer the peel into the watch glass containing the safranin solution. Let this remain in the Safranin solution for 30 seconds, so that the peel is stained.

Answered by sharmaaayush4455
1

Answer:

Here is your answer

To prepare stained temporary mount of onion peel:-

Procedure:-

1. Tear a fleshy leaf of onion & collect a piece of the peel.

2. Spread the peel carefully on a slide.

3. Put a few drops of Janus green B stain on the peel. Allow it to stain for 5-10 min.

4. Wash the extra stain with the help of distilled water. Be careful so that entire stain doesn't get washed off.

5. Mount the cells in a drop of distilled water.

6. Use coverslip to cover the peel. Observe the slide under high power of a compound microscope.

The cells can alternatively to be mounted in the stain itself. A few air bubbles remaining inside the cover glass give a background stain that makes the viewing easy. (Note:-Adjust the microscope lens in sequence from Low power to high power.)

hope it's helpful

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