how to preserve chloroform and why?
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Chloroform needs to be stored in a cool, dry, well-ventilated place, out of direct light. Our choice of storage container is likely to be influenced by how long we intend to store it for, but an amber glass bottle with a ptfe-lined cap is probably best suited for longer storage.
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It is very important to prevent the oxidation of chloroform. This can be done as follows-
(a) Chloroform should be stored in dark colored bottle to cut off the light.
(b) The bottle containing chloroform should be filled up to the stopper and closed tightly to avoid the presence of air inside the bottle.
(c) A small quantity of ethanol (1%) is added with CHCl3 at the time of packaging. Ethanol converts phosgene gas into non-toxic and non volatile neutral ethyl carbonate.
COCl2 + 2C2H5OH = (C2H5)2CO3 + 2HCl
Chloroform (trichloromethane) and saturated thymol (5-methyl-2-[1-methyl ethyl]phenol) were used as biocides to preserve synthetic and real throughfall water samples for up to 50 days.
(a) Chloroform should be stored in dark colored bottle to cut off the light.
(b) The bottle containing chloroform should be filled up to the stopper and closed tightly to avoid the presence of air inside the bottle.
(c) A small quantity of ethanol (1%) is added with CHCl3 at the time of packaging. Ethanol converts phosgene gas into non-toxic and non volatile neutral ethyl carbonate.
COCl2 + 2C2H5OH = (C2H5)2CO3 + 2HCl
Chloroform (trichloromethane) and saturated thymol (5-methyl-2-[1-methyl ethyl]phenol) were used as biocides to preserve synthetic and real throughfall water samples for up to 50 days.
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