How to produce recombinant DNA vaccine against Hepatitis B using animal cell culture technique?
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Vaccination is one of the most successful and cost-effective public health interventions of all. It has eradicated smallpox, lowered the global incidence of polio by 99% since 1988 and achieved dramatic reductions in diseases such as measles, diphtheria, whooping cough (pertussis), tetanus and hepatitis B. “Vaccination is the 20th century’s greatest public health triumph after improved access to clean, safe drinking water.” (1)
Prior to the advent of cell culture, viruses could be propagated only on whole organisms, animal or plants. Whole organisms could include the natural host and laboratory animals such as chicken embryonated eggs, rabbits, mice, rats and others. The development of cell culture techniques in the 1950’s opened the door to the manufacturing of a wide range of biological pharmaceutical products at industrial scale.
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