How to quantitate chlorophyll content from green algae?
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Answer:
A chlorophyll removing method for microalgae is disclosed. Chlorophyll can be removed by mixing microalgae powder or wet microalgae mud with a decolouring liquid according to a concentration (a massic volume ratio) of 0.5-5.0%, and reacting at 40-70 DEG C for 0.5-3.0 h. A preparing method of the decolouring liquid includes mixing a low-carbon alcohol and an alkali solution having a concentration of 0.5-2.5% according to a certain volume ratio. The chlorophyll removing method is efficient, simple, easy, safe and low in cost, can remove high-content chlorophyll from the microalgae, and effectively solves the chlorophyll contamination problem of microalgae products. A decolouring waste solution can be used for preparing sodium copper chlorophyllin. The microalgae powder or the wet microalgae mud the chlorophyll of which is removed can be used for extraction preparation of microalgae oil, proteins, polysaccharides and carotenoid, and related products can be used for developing healthcare products, cosmetics, biological energy, feed, baits, and the like.