Biology, asked by Boom1326, 11 months ago

How to separate plasmid dna from chromosomal dna in gel?

Answers

Answered by nirmiti06
0

Answer:

After lysis, a neutralizing solution is added causing the denatured genomic DNA to precipitate, while the circular, covalently closed plasmid DNA reanneals, remaining in solution. The plasmid DNA is then separated from precipitated cellular debris and genomic DNA by centrifugation.

Answered by rithvik301
0

Answer:

Explanation:

After lysis, a neutralizing solution is added causing the denatured genomic DNA to precipitate, while the circular, covalently closed plasmid DNA reanneals, remaining in solution. The plasmid DNA is then separated from precipitated cellular debris and genomic DNA by centrifugation.

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