How to subculture bacteria from mother culture for long time storage?
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Before you can grow bacteria, you'll need to prepare sterile culture dishes. A 125ml bottle of nutrient agar contains enough to fill about 10 petri dishes. Water Bath Method – Loosen the agar bottle cap, but do not remove it completely. Place the bottle in hot water at 170-190 °F until all of the agar is liquid.
u can use glycerol for preserving it longer
u can use glycerol for preserving it longer
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