Question

how to use paraformladehyde fixed tissue for cryosection

Answer 1

hello friend here is your answer
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I did crysections for muscle tissue that fixed in PFA and cryo protected in sucrose and embedded in ... I currently use OCT and cryostat setted on -20 / -25°C, it works fine.
i hope it will help you

Answer 2

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Prepare frozen tissue sections (steps 1-8): Slowly place the base mold containing the tissue block into liquid nitrogen till the entire tissue block is submerged into liquid nitrogen to ensure tissue is frozen completely. Store the frozen tissue block at -80°C until ready for sectioning.