how u will prepare 4% paraformaldehyde solution?
Answers
Answer:
Reagents Required
Deionized H2O
HCl (Dilute)
NaOH (1 N)
Paraformaldehyde powder
1X PBS: 0.145 M NaCl, 0.0027 M KCl, 0.0081 M Na2HPO4, 0.0015 M KH2PO4, pH 7.4
Materials
Filter units
Glassware and stir bar (dedicated for formaldehyde solution)
Gloves and eye protection
Hot plate with magnetic stirrer
Thermometer
Ventilated hood
Procedure
For 1 L of 4% Formaldehyde, add 800 mL of 1X PBS to a glass beaker on a stir plate in a ventilated hood. Heat while stirring to approximately 60 °C. Take care that the solution does not boil.
Add 40 g of paraformaldehyde powder to the heated PBS solution.
The powder will not immediately dissolve into solution. Slowly raise the pH by adding 1 N NaOH dropwise from a pipette until the solution clears.
Once the paraformaldehyde is dissolved, the solution should be cooled and filtered.
Adjust the volume of the solution to 1 L with 1X PBS.
Recheck the pH, and adjust it with small amounts of dilute HCl to approximately 6.9.
The solution can be aliquoted and frozen or stored at 2-8 °C for up to one month.
Explanation:
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Answer:
For 1 L of 4% Formaldehyde, add 800 mL of 1X PBS to a glass beaker on a stir plate in a ventilated hood. Heat while stirring to approximately 60 °C. Take care that the solution does not boil.
Add 40 g of paraformaldehyde powder to the heated PBS solution.
The powder will not immediately dissolve into solution. Slowly raise the pH by adding 1 N NaOH dropwise from a pipette until the solution clears.
Once the paraformaldehyde is dissolved, the solution should be cooled and filtered.
Adjust the volume of the solution to 1 L with 1X PBS.
Recheck the pH, and adjust it with small amounts of dilute HCl to approximately 6.9.
The solution can be aliquoted and frozen or stored at 2-8 °C for up to one month.