Question

how u will prepare 4% paraformaldehyde solution?​

Answer 1

Answer:

Reagents Required

Deionized H2O

HCl (Dilute)

NaOH (1 N)

Paraformaldehyde powder

1X PBS: 0.145 M NaCl, 0.0027 M KCl, 0.0081 M Na2HPO4, 0.0015 M KH2PO4, pH 7.4

Materials

Filter units

Glassware and stir bar (dedicated for formaldehyde solution)

Gloves and eye protection

Hot plate with magnetic stirrer

Thermometer

Ventilated hood

Procedure

For 1 L of 4% Formaldehyde, add 800 mL of 1X PBS to a glass beaker on a stir plate in a ventilated hood. Heat while stirring to approximately 60 °C. Take care that the solution does not boil.

Add 40 g of paraformaldehyde powder to the heated PBS solution.

The powder will not immediately dissolve into solution. Slowly raise the pH by adding 1 N NaOH dropwise from a pipette until the solution clears.

Once the paraformaldehyde is dissolved, the solution should be cooled and filtered.

Adjust the volume of the solution to 1 L with 1X PBS.

Recheck the pH, and adjust it with small amounts of dilute HCl to approximately 6.9.

The solution can be aliquoted and frozen or stored at 2-8 °C for up to one month.

Explanation:

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Answer 2

Answer:

For 1 L of 4% Formaldehyde, add 800 mL of 1X PBS to a glass beaker on a stir plate in a ventilated hood. Heat while stirring to approximately 60 °C. Take care that the solution does not boil.

Add 40 g of paraformaldehyde powder to the heated PBS solution.

The powder will not immediately dissolve into solution. Slowly raise the pH by adding 1 N NaOH dropwise from a pipette until the solution clears.

Once the paraformaldehyde is dissolved, the solution should be cooled and filtered.

Adjust the volume of the solution to 1 L with 1X PBS.

Recheck the pH, and adjust it with small amounts of dilute HCl to approximately 6.9.

The solution can be aliquoted and frozen or stored at 2-8 °C for up to one month.