how we can done screening of plant for heat stress ??
Answers
Heat stress often is defined as where temperatures are hot enough for sufficient time that they cause irreversible damage to plant function or development. The methods include a protocol for screening heat tolerance of rice at the seedling stage; young seedlings were exposed to heat stress and the test takes 4–5 weeks and involves the visual scoring of symptoms which allows hundreds of seedlings to be evaluated in a short time. The visual screening method was extensively validated through laboratory, glasshouse, and field-based experiments. We also adapted a protocol for screening heat tolerant mutant lines at the flowering (reproductive) stage that has been specifically adjusted for a mutation breeding program. Here, plants were treated from the first day of anthesis at different temperatures and spikelet fertility at maturity was determined as a parameter to assess the heat tolerance of the selected genotypes.
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