How will nucleus stain?
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HAEMATOXYLIN IS A NUCLEAR STAIN.
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- The nucleus of the cell is a membrane-bound organelle that includes the nuclear envelope, nucleoli, and nuclear lamina and is the site of gene expression.
- Nuclear structure can be selectively visualized by staining nuclear proteins or directly staining nucleic acids. Cell-permeant nucleic acid stains make it possible to stain live cells or tissues with minimal processing to reveal the natural location of cells in tissues and provide a means to follow nuclear changes
- The nucleus is composed of DNA, basic proteins, non-basic proteins, RNA, lipids and minerals. The most important basic proteins are the histone proteins which are required for coiling and packaging of DNA present in chromatin material.
- The histone proteins are rich in basic amino acids like lysine and arginine and hence have a positive charge naturally. Haematoxylin stains basophilic substances. Hence, hematoxylin is commonly used to stain the nucleus.
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