how will you determine whether the given food items contain protein or not ?
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Following steps are used to test if a food item contains protein.
1) Take a small quantity of the food items to be tested.
2) Grind/mash the food item.
3) Put the food item into a test tube.
4) Add 10 drops of water and shake the test tube.
5) Add two drops of copper sulphate solution to the test tube, using a dropper.
6) Add 10 drops of caustic soda solution using a dropper and shake the test tube.
7) Let the test tube rest in a test tube stand for a few minutes.
8) Observe the colour of the mixture.
9) Violet colour indicates the presence of proteins in the tested food item.
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Grind or mash a small quantity of the food item. Put some of this in a clean test tube, add 10 drops of water to it and shake the test tube. ... A violet colour indicates presence of proteins in the food item .
Take the given food sample, prepare the extract of the given food sample. Then add the aqueous copper sulphate to the prepared food extract. A solution turns violet in colour confirms the presence of protein in the foodstuff.
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