How will you grow bread moulds ? Show me
with an experiment?
Answers
Answer:
1. Using the sticky labels and the marker pen label the bags. Mark 5 bags as ‘A’, 5 as ‘B’ and 5 as ‘C’. You also need to label each set of bags 1 to 5.
2. Cut the bread into 10 x 10 squares using the chopping board and knife.
3. Inoculate the bread thoroughly with the mold solution. Try to coat each slice with a similar amount of the culture although this can be difficult.
4. Put one slice of this bread into each bag and seal the bags tightly.
5. Put the 5 ‘A’ bags into the freezer, the 5 ‘B’ bags into the refrigerator and the 5 ‘C’ bags somewhere safe in a warm room. Because the bags in the freezer and fridge will not be getting much light it is best to cover the ‘C’ bags to make sure that light is a constant.
6. Every 24 hours, preferably at exactly the same time every day, using the plastic grid, count the number of square centimeters of mold on each slice of bread. If the mold covers more than half a square, count it as 1cm, if less than half a square, count as 0 cm. You must never open the bags.
7. You should repeat these counting processes for 10 days or until there are significant measurable results.
8. Keep a careful note of your results for each slice of bread for the entire duration of the experiment. You can even take pictures or draw the slices if you want to be really scientific!
9.Average the results for sample types A, B and C.
10. Once you have finished, throw out all of the bags without opening them.