How will you test the presence of chlorophyll in the leaves of the plant?
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Only the parts that were green become blue-black with iodine solution, showing the importance of chlorophyll in photosynthesis. A plant can be 'de-starched' by leaving it in the dark for a few hours.
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- Add the leaf to boiling ethanol in a water bath for a few minutes (the boiling ethanol dissolves the chlorophyll and removes the green colour from the leaf - it turns white so it is easy to see the change in colour) add iodine solution from a dropping pipette.
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