How will you test the presence of protein in a food sample?
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Answer:
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Explanation:
Take a small quantity of the food items to be tested.
Grind/mash/paste/powder the food item.
Put the food item into a test tube.
Add 10 drops of water and shake the test tube.
Using a dropper add two drops of copper sulphate solution to the test tube.
Add 10 drops of caustic soda solution and shake the test tube.
Observe the colour of the mixture.
Violet colour indicates the presence of proteins in the tested food item.
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Biuret solution is used to identify the presence of protein. Biuret reagent is a blue solution that, when it reacts with protein, will change color to pink-purple.
(Refer the above image for more details.)
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