How would you get sample for karyotypic analysis in allium cepa and allium sativum in a given locality?
Answers
Explanation:
Onion chromosomes (Allium cepa L.; 2n=2x=16) were analyzed for karyotype analysis using aceto-orcein staining technique, and classified due to their lengths from long to short arms whether satellite exists or not.The length of arms appeared to be very close to each other and got more force their identification. The length of long arms showed a variation from 5 to 14 μm while short arms have 2 to 10 μm at the early and late stages of mitotic metaphase. The reason of this variation comes from the stages which observed as late prophase in mitosis. This different chromosome length also was affected in regard to the satellite position seen on long chromosome. The folding rate changed between 1.01 to 2.16 due to the smallest total length of chromosomes.
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