How would you prepare a protein free filtrate (PFF)?
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Answer: First of all take 2ml of blood.
Then shift this blood to a container containing potassium oxalate.
Then dilute the blood with distilled water. Add 14ml of Dist water.
Then add 2ml of 10 percent sodium tungstate.
Then add 2/3 normal H2SO4.
Color of solution will change from dark red to dark brown.
Filter this solution using Whatman filter paper.
After filtration, you will get colorless protein free filtrate.
Explanation:this is one method pretty sure others exist too
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