How would you use boiled seedlings to show that any change of gas was due to respiration?
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The seeds are boiled with an antiseptic to avoid bacterial decay. Solution: (i) Soda Lime – To absorb any carbon dioxide released by the seeds (ii) Limewater – Change in the color of limewater to milky water indicates the presence of carbon dioxide, and its source being germinating seeds only.
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As the seeds respire, they are taking in oxygen and respiring out carbon dioxide, but the carbon dioxide is absorbed by the calcium hydroxide. As a result, the amount of air in the sealed test tube actually decreases and water rises in the test tube.
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