Question

HPLC principle and INSTRUMENTATION
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Answer 1

Answer:

The separation principle of HPLC is based on the distribution of the analyte (sample) between a mobile phase (eluent) and a stationary phase (packing material of the column). Depending on the chemical structure of the analyte, the molecules are retarded while passing the stationary phase.

Explanation:

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Answer 2

Answer:

• HPLC principles ... It is a technique by which a mixture sample is separated into components for identification , quantification and purification of mixtures.
• HPLC instrumentation is typical made up of nine basic components: mobile/ phase solvent reservoir, solvent delivery system, sample introduction device, etc...