Question

. Hydrolytic driving force: • The hydrolysis of pyrophosphate to orthophosphate is important in driving forward biosynthetic reactions such as the synthesis of DNA. This hydrolytic reaction is catalyzed in Escherichia coli by a pyrophosphatase that has a mass of 120 kd and consists of six identical subunits. For this enzyme, a unit of activity is defined as the amount of enzyme that hydrolyzes 10 umol of pyrophosphate in 15 minutes at 37°C under purified enzyme has a V max of 2800 units per standard assay conditions. The milligram of enzyme. (a) How many moles of substrate are hydrolyzed per second per milligram of enzyme when the substrate concentration is much greater than K M ? (b) How many moles of active site are there in 1 mg of enzyme? Assume that each subunit has one active site. (c) What is the turnover number of the enzyme? .​

Answer 1

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