I) Which of the following stain is used for preparing the temporary stained mount onion peeled.
II) Write down the procedure (experiment) of onion peel
III) What are the function of parenchyma tissues?
IV) In practical class your teacher shows you two slides. One of these is that of plant cell you are asked to identify the plant and animal cells. Name at least two characters which you observe to distinguish them.
V) What is the use of glycerine in preparation of slide of onion peel and which stains used in it ?
There are some bundles of questions please answer them fast but please answer all questions who will answer all questions I will mark him brailiest
Answers
a) Methylene blue
b) Procedure:
1. Take an onion and remove its outermost peel.
2. Now cut a small part from an inner scale leaf with the help of a blade.
3. Separate a thin, transparent peel from the convex surface of the scale leaf with the help of forceps.
4. Keep this peel in a watch glass containing water?
5. Add two drops of safranin stain in the watch glass to stain the peel.
6. Take a clean slide and put a drop of glycerine in the centre of the slide.
7. With the help of a brush and needle transfer the peel on the slide. Glycerine prevents the peel from drying up.
8. Carefully cover it with a coverslip and avoid any air bubble from entering interring the coverslip.
9. Remove any excessive glycerine with a filter paper.
10. Observe the prepared mount of the peel under the low and high magnification of a compound microscope.
c) Parenchyma forms the bulk of plant ground tissue, where they may be specialised to function in photosynthesis, storage, or transport. Parenchyma is integral to vascular tissue, where it provides a route of exchange for materials within and between the xylem and the phloem.
d) You are observing two unlabeled cells a plant and an animal cell- through a microscope. what cell parts can you look for to determine. which is the plant cell and which is the animal cell?
There are a few key structural differences between plant cells and animal cells.
Below are a few of the main ones:
Plant cells are generally larger than animal cells.
Only animal cells have lysosomes (however, recent, and controversial, research suggests that some plant cells may have lysosomes), and only plant cells have chloroplasts.
While plants cells have a rigid cell wall, animal cells have a thin and flexible plasma membrane.
Plants cells have a large central vacuole and animal cells have many small vacuoles.
e) Glycerine is used for staining the specimen which is present in our glass slide.
It is considered as a mounting agent while making a slide of onion peel
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