Biology, asked by caderioholland03, 9 months ago

Identify the independent variable in the experiment described. Identify the plasmid that was used as a negative control for luciferase activity. Justify including the plasmid with the non- CD3γ active promoter in the experiments.

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Answered by cgonzalezsoto03

41

Answer:

The independent variable is the the plasmid sequences, which contain different amounts of CD3y regulatory sequence. The parent plasmid served as the negative control for the luciferase activity. The aim of the experiment is to analyze regulatory sequences present with the CD3y gene, which might influence the expression of the gene. The plasmid with then non-CD3y active promoter shows the normal activity of the luciferase gene, since it not being regulated and completely free to manifest, which can also help identify which the positive and negative plasmids.

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