Question

Identify the plasmid that must contain the CD3γ core promoter sequence but the fewest or no negative regulatory sequences. Based on the data in Figure 2, describe the most likely cause of the variation in luciferase activity among the cells that contain plasmids pCD3γ-419 , pCD3γ-309 , pCD3γ-239 , and pCD3γ-199 . Calculate the approximate percent increase in luciferase activity between cells containing plasmid pCD3γ-59 and cells containing plasmid pCD3γ-149 . Round to the nearest whole number.

Answer 1

Answer:

A luciferase assay is used to determine if a protein can activate or repress the expression of a target gene. If the protein is able to upregulate the transcription of the target gene, the cells will express luciferase. If the protein downregulates transcription, the cells will express