Question

Identify the reason for the selection of dna polymerase from thermus aquaticus for polymerase chain reaction

Answer 1

Enzymes get denatured at high temperatures.
Thermus aquaticus is a thermoacidophile (an archaebacteria). It lives in very high temperatures. Thus the enzymes produced by it will also be active at very high temperatures ie., wont get denatured at high temperatures.
Therefore dna polymerase also called Taq polymerase(as it is obtained from Thermus aquaticus) is ideal .(we usuallu heat dna to denature it ie., to get 2 starnds from the double stranded dna during polymerisation).......


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