Question

If 1ml of an unknown sample of protein is diluted to 10ml, and a  0.5ml portion of the diluted sample is found to contain 50ug of protein     when assayed for protein using the lowry method. What is the concentration of protein in ug/ml in the original sample ?​

Answer 1

Answer:

If 1ml of an unknown sample of protein is diluted to 10ml, and a  0.5ml portion of the diluted sample is found to contain 50ug of protein     when assayed for protein using the lowry method. What is the concentration of protein in ug/ml in the original sample ?​

Explanation:

Answer 2

Given: 1ml of an unknown sample of protein is diluted to 10ml, and a  0.5ml portion of the diluted sample is found to contain 50ug of protein when assayed for protein using the Lowry method

To Find: the concentration of protein in ug/ml in the original sample

Explanation:

Let the amount of protein present in the original sample be y ug.

The concentration of protein in original sample: y ug/ ml

When diluted to 10 ml, the concentration will be: y ug/ 10 ml or 0.1 y/ ml

0.5 ml of the above solution is taken. It would contain:

$0.1 y\times 0.5 = 0.05 y$

From assay. the protein was found: 50 ug

$0.05 y = 50 ug\\y = \frac{50}{0.05} \\y = 1000 ug$

Hence, the concentration of protein in the original sample is 1000ug/ml