if 2.5m of acetone is present in 110ml of its aqueous solution calculate the concentration of solution?
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Essential Maths for Medics and Vets Reference Materials Module 2. Amount and Concentration.
Creative Commons Attribution Non-commercial Share Alike Author Dr J A Koenig
Page 1 of 7
2 Amount and concentration: making and diluting solutions
2 Amount and concentration; making and diluting solutions.........................................................1
2A Rationale.............................................................................................................................1
2B Distinguishing between amount and concentration, g and %w/v..........................................1
2C Distinguishing between amount and concentration, moles and molar...................................2
2D Practice converting g/L to M and vice versa........................................................................3
2E Diluting Solutions ...............................................................................................................5
2F Practice calculating dilutions ...............................................................................................6
Summary of learning objectives................................................................................................7
2A Rationale
Biological and biochemical investigations rely completely upon being able to detect
the concentration of a variety of substances. For example, in diabetics it is important
to know the concentration of glucose in the blood and you may also need to be able to
calculate how much insulin would need to be dissolved in a certain volume of saline so
as to give the right amount in a 1ml injection volume. It is also vitally important that
you perform these calculations quickly, reliably and accurately.
This section aims to help you understand how to go about doing these calculations and
to give you plenty of practice so that you can do them quickly and reliably.
2B Distinguishing between amount and concentration, g and %w/v
The amount of a substance is often measured by how much it weighs – i.e. in g or mg
or kg etc.
The concentration is the amount divided by the volume it is dissolved in.
The concentration is the amount divided by the volume it is dissolved in. amount concentration
The concentration is the amount divided by the volume it is dissolved in. amount concentration volume
=
For example you might dissolve
0.9g of sodium chloride in 100mL of water.
Sometimes this is referred to as 0.9% w/v since it is 0.9g weight divided by 100mL
volume and “w/v” stands for weight per volume.”
The concentration of this solution could be described as
0.009 g/mL or 9 mg/mL.
0.009 g/mL or 9 mg/mL. Alternatively you could notice that if 0.9g is dissolved in 100mL, then 9g would be
0.009 g/mL or 9 mg/mL. Alternatively you could notice that if 0.9g is dissolved in 100mL, then 9g would be dissolved in 1000ml (=1L) and the concentration could also be written as