Question

If a 4kb fragment of dna is to be amplified from 8kb plasmid then,how many copies are obtained at the end of 30 pcr cycles approximately?

Answer 1

there are several types of malnutrition diseases.

mostly malnutrition is of three types :

1. calorie malnutrition

2. protein malnutrition

3. protein calorie malnutrition.

1.kwashiorkor disease :

this is due to protein deficiency in diet.

body parts become swollen due to accumulation of water in the intercellular spaces.

very poor muscle development, sonilex, fluffy face difficult to eat, diarrhoea , dry skin are the symptoms of these disease.

2.marasmus :

this is due to deficiency of both proteins and calories.

generally this disease occurs when there is an immediate second pregnancy or repeated child Births.

lean and weak, less development muscles, dry skin etc are symptoms of this disease.

3.obesity :

this is due to overeating and excess of calories intake.

it is a big health hazard . obase children when grow, they will be target of many diseases like diabetes comma cardiovascular, NL, glad bladder and problems.

how this answer is a clear and useful to you.

Answer 2

Answer:*Rule in amplification*

Size doesn't affect the PCR. it's following the formula

2^n

n is the no. of cycles.

2^30

In formula *n* replace by 30

2^30=1073741824

Explanation:

1 billion is correct answer