Question

If a sample has a DNA of size 2kb and 6kb while
the molecular marker denotes 4kb band as it's
first band where will be the 6kb band of DNA?
A. Above the band of another DNA sample
B. Above the band of marker
In between the band of marker and
another band of DNA sample.
D. Both A and B
​

Answer 1

Answer:You can see the bands of any size of DNA in whatsoever %age of agarose gel you will use. But optimum resolution can only be achieved by using the optimum %age of agarose. I am sharing a table of recommended % agarose and optimum resolution of linear dna with you for your convenience... (source: promega)

0.5% 1,000 - 30,000 bp

0.7% 800 - 12,000 bp

1.0% 500 - 10,000 bp

1.2% 400 - 7,000 bp

1.5% 200 - 3,000 bp

1.8% 100 - 2,000 bp

2.0% 50 - 2,000 bp

You should always pick the gel percentage according to the smallest linear dna fragment for better resolution... and in your case it is 100 bp... so 1.5 - 1.8% agarose gel would suffice your need.

All the best

Explanation: