Question

If alien DNA is introduced using pvu I in PBR322 then transformant will grow on
(A) Tetracycline
(B) Ampicillin
(C) Both (A) and (B)
(D) Either (A) or (B)
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Answer 1

Answer:

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Answer 2

Answer:

(A) Tetracycline

Explanation:

If alien DNA is introduced using pvu I in PBR322 then transformant will grow on tetracycline. Because pvu I is present at ampicillin resistance site. If the alien DNA is added there, the ampicillin resistance site will become inactive and hence cannot grow on Ampicillin. But the genes remain the same without the alien DNA interruption at tetracycline resistance site, hence the bacteria will grow on tetracycline.

Good question Btw!!