Question

If E.coli is allowed to grow for 40 minutes in a medium containing N15 ,then the number of n14/n14 containing DNA would be

Answer 1

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★ As we know E.coli replicates after every 20 mins.

★ And each strand of DNA seperate out to combine with newly synthesis strand.

★ Here,

after 20 mins ratio of N14 : N 15 DNA strand is 0:2 (both strands are radioactive )


★ N15 (means radioactive medium )

★ After 40mins ratio of N14 : N15 is 1:1 ( 2 non- radioactive and 2 radioactive strands )

Happy to help !

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