if the GC content of DNA molecule is 60%.what are the percentage od G C A T bases?
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If the GC content of a DNA molecule is 56 percent, what are the percentages of the four bases (A, T, G, and C) in this molecule?
If the GC content is 56 percent, then, because G = C, the content of G is 28 percent and the content of C is 28 percent. The content of AT is 100 − 56 = 44 percent. Because A = T, the content of A is 22 percent and the content of T is 22 percent.
2. Describe the expected pattern of bands in a CsCl gradient for conservative replication in the Meselson-Stahl experiment. Draw a diagram.
Refer to Figure 8-13 for an additional explanation. In conservative replication, if bacteria are grown in the presence of 15N and then shifted to 14N, one DNA molecule will be all 15N after the first generation and the other molecule will be all 14N, resulting in one heavy band and one light band in the gradient. After the second generation, the 15N DNA will yield one molecule with all 15N and one molecule with all 14N, whereas the 14N DNA will yield only 14N DNA. Thus, only all 14N or all 15N DNA is generated, again, yielding a light band and a heavy band:
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Because A = T, the content of A is 22 percent and the content of T is 22 percent
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