Question

If the population density of a given sample is 330,000 cfu/ml, approximately how many colonies will grow when 0.1ml of a 10-3

Answer 1

Lungs, brains, spleens, livers and kidneys were aseptically removed and approximately half of each organ was weighed and homogenized in 1 ml of sterile saline. Serial 10-fold dilutions of the homogenates were inoculated on SDA, incubated at 35 C and examined daily for 3 days. The number of CFU/g-1 of tissue was calculated for each sample.

1. Is it necessary we weighed organ?

2. What is exactly meaning of Serial 10-fold dilutions?

3. How can we calculate CFU? Any protocol or methods for this?

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