If you call I is allowed to grow for 80 minutes in any age 14 CL 15 isotope medium then what would be the proportion of a hybrid and the light density DNA molecule
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Explanation:
E coli bacteria divides in every 20 min so in 80 min it will be four generations.
N15 isotope is present in the environment than shifted in N14 environment, so it will result in the presence of N15 and N14 strands in DNA.
In the second generation, half will have the previous hybrid bacteria and half will have the light bacteria.
In the third generation, hybrid generation will again cut half, this time 25% hybrid and 75% light
In fourth generation, 16 copies will be hybrid and 14 will be light
Meselson and Stahl found that DNA of the first generation was hybrid or intermediate (and). It settled in caesium chloride at a level higher than the fully labeled DNA of parent bacteria (). The second generation of bacteria after 40 minutes, contained two types of DNA, 50% light() and 50% intermediate(). The third generation of bacteria after 60minutes contained two types of DNA, 25%intermediate (N15 N14) and 75% light (N14 N14) in 1:3 ratio. The fourth generation after80 minutes contained 12.5%N15 N14 and 87.5% N14N14 DNA in 1:7 ratio.
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