Biology, asked by abdullahchattha77, 4 months ago

if you given explant to culture in artificial nutrient medium then how will you culture it? describe all step one by one.​

Answers

Answered by bagchinadashree
0

Answer:

To understand a procedure that is often used to propagate many plants of the same genetic background.

To understand the importance of sterile techniques

Explanation:

:

1 vial of Murashige Skoog (MS) medium. (If you wish to make up your own growing medium you could use the recipe for the Murashige medium given at the end of this section.)

1 L sterile distilled water

8 g of agar/L

30 g sucrose/L

1.5-L or 2-L container in which to prepare the growth medium

Small amounts of 1M NaOH and 1M HCl to adjust the pH of the medium

30 flat-bottom culture tubes with closures

Glass aquarium or box lined with plastic

Plastic sheet to cover the top of the aquarium

Adhesive tape

10% bleach in a spray bottle

70% alcohol in a spray bottle

Forceps or tweezers

Gloves

Cutting equipment such as a scalpel blade or razor blade

2 bottles of sterile distilled water (purchase at the grocery store)

Pressure cooker

Your chosen plant (cauliflower, rose, African violet, or carnation)

Paper towel for cutting on, or sterile petri dishes if available

Beaker or container in which to wash the plant material

Detergent-water mixture - 1 ml detergent per liter of water

Bleach sterilizing solution - dilute commercial bleach (5-6% sodium hypochlorite) to a final concentration of 1-2% sodium hypochlorite in distilled water in a large beaker or jar.

2 or 3 beakers or containers of sterile water

A well-lit area away from direct sunlight or use full-spectrum gro-lights

Hormones such as BAP (benzylaminopurine) and NAA (naphthalene acetic acid) to stimulate growth and root development, respectively. (Commercial rooting hormone solutions and powders are also available from hardware stores.)

Answered by mousam8673
0

Answer:

  • As part of plant tissue culture, a small part of plant tissue is taken and used from the mother plant (donor plant) and grown (cultured) on a nutrient medium in sterile containers.
  • The whole plant can be re-generated by changing the composition of the medium and other conditions like temperature and light (environmental conditions).
  • Finally, the plant is re-produced from single plant tissue which will be 100% identical in all aspects.
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