If you have to load 20 ug of DNA in a gel, calculate the volume required from each sample
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Explanation:
Hello, I am facing some struggles with maths, gel elecrophoresis and my DNA samples. I need to use 1uL of 6x loading dye and MQ water to equalize DNA samples for GE. The average DNA sample load for GE well is 5 uL + 1 uL of loading dye + 4 uL MQ water, but I want them all to be even, like, for instance, 3,2 will need 1,8 MQ to reach the equality.
I got NanoDrop data (the concentrations are like 16,4 ng/uL, 13,4... and etc.), but I cannot reach my goal described above due to my problems with the logical chain of actions.
My DNA samples were washed with the elution buffer of 50 uL. I am not even sure if I am calculating the total amount correctly as well (it is a basic formula, but I guess that I am confused by the results I keep having, so I hope someone could help me to understand my issues and shed some light on the matter).
Thank you!