if you reach in hematology laboratory, and your senior technician asks you to perform tlc counting by neubaur's method then which technique you will use. explain.
Answers
Explanation:
White blood cells develop from the stem cells in the bone marrow.
Stem cells differentiate into:
Granulocytic series cells. The granulocytes got the name due to the presence of distinct granules in the cytoplasm of these cells.
The life span of leucocytes varies from 13 to 20 days; after that, these cells are destroyed in the lymphatic system and may be excreted from the body in feces.
Leucocytes fight infections and defend the body by a process called phagocytosis.
WBC serves as an excellent indicator of the various disease process.
Non-Granulocytic cells.
The granulocytic series consists of:
polymorphonuclear leucocytes (Neutrophils).
Eosinophils.
Basophils.
Monocytes.
Neubauer's method is used for the purpose of total leucocyte counts in hematology laboratories.
- To determine if you have leucocytosis (an increase in the number of white blood cells to more than 11000/mm³) or leucopenia (a decrease in the number of white blood cells to less than 1500/mm³), a total white blood cell (WBC) count is performed.
- To evaluate how well the bone marrow is working, the Total Leucocyte Count (TLC) is also performed.
- It is practically impossible to count so many White cells under the microscope.
- Therefore, the Leucocytes are counted using a particular type of chamber called a hemocytometer or Neubauer's chamber, created for the counting of blood cells in the specimen.
- To do this, the blood sample is diluted (often at a ratio of 1:20) using WBC dilution fluid, also known as Turk's Fluid, which preserves, fixes, and stains white blood cells while lysing red blood cells.
- Since the Turk's fluid is isotonic with white blood cells, it does not harm them.
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