Question

Imagine you are generating a site-directed mutant of a protein in which a given residue is replaced by another
residue. Which one of the following substitutions will result in the highest isoelectric point of the mutant
compared to the wild type? (X → Y means residue X in the wild type is replaced by residue Y in the mutant).
a. Asp → Arg
b. Arg → Asp
C. Tyr Lys
d. Gly → Arg

please explain how to solve.​

Answer 1

Answer:

a is the answerSite-directed mutagenesis of an amino acid residue in the bacteriophage P2 ogr protein implicated in interaction with Escherichia coli RNA polymerase

Answer 2

Answer:

a

Explanation:

Site-directed mutagenesis is a molecular biology method that is used to make specific and intentional changes to the DNA sequence of a gene and any gene products. . In case of protein the mutation may be a single amino acid change (a point mutation), multiple amino acid changes, deletion, or insertion.

So when one amino acid residue(x)i.e wild type, is replaced by another amino acid residue(y) i.e mutant, the substitution which will result in the highest isoelectric point (pI) of mutant (y) then wild type (x) is option(a) as (y)ie.Arginine has highest pI 10.76 compared to (x) ie.Aspartate having lowest pI 2.77.