Immunoelectrophoresis principle and applications
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its gaurav,
the answer is ,
IMMUNOELECTROPHORESIS
This is mainly used for separating protein portions from serum samples. Immunoelectrophoresis mainly consists of two methods namely immunodiffusion and electrophoresis.
Electrophoresis is defined as the migration of charged particles under the influence of an electric field which facilitates the separation of a mixture of a compound into its component parts. In immunodiffusion the separated components (antigens) obtained by electrophoresis are allowed to diffuse through agar.
APPLICATIONS:-
1).to test protein present in uterine and body fluids
2).to test normal and abnormal serum proteins
3).for identification of serum protein
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i hope it will help you
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its gaurav,
the answer is ,
IMMUNOELECTROPHORESIS
This is mainly used for separating protein portions from serum samples. Immunoelectrophoresis mainly consists of two methods namely immunodiffusion and electrophoresis.
Electrophoresis is defined as the migration of charged particles under the influence of an electric field which facilitates the separation of a mixture of a compound into its component parts. In immunodiffusion the separated components (antigens) obtained by electrophoresis are allowed to diffuse through agar.
APPLICATIONS:-
1).to test protein present in uterine and body fluids
2).to test normal and abnormal serum proteins
3).for identification of serum protein
thnx for this wonderful question
i hope it will help you
plzz mark as brainliest dear plzzz plzzz plzzzzzzzzz
Answered by
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Immunoelectrophoresis : Biochemical methods for separation and characterization of proteins based on electrophoresis and reaction with antibodies.
Principle: In immunoelectrophoresis, the antigen mixture is first electrophoresed to separate its constituents by charge. The antiserum containing the antibodies added into the troughs diffuses with a plane front to react with the antigens. Due to diffusion, density gradient of the antigen and antibody are obtained and at a specific antigen/antibody ratio (equivalence point) huge macromolecules are formed. They form a visible white complex which precipitates as arcs in the gel. The arc is closer to the trough at the point where the antigen is in highest concentration. The method is very specific and highly sensitive because distinct zones are formed. In this method it is important that the ratio between the quantities of antigen and antibody be proper.
Principle: In immunoelectrophoresis, the antigen mixture is first electrophoresed to separate its constituents by charge. The antiserum containing the antibodies added into the troughs diffuses with a plane front to react with the antigens. Due to diffusion, density gradient of the antigen and antibody are obtained and at a specific antigen/antibody ratio (equivalence point) huge macromolecules are formed. They form a visible white complex which precipitates as arcs in the gel. The arc is closer to the trough at the point where the antigen is in highest concentration. The method is very specific and highly sensitive because distinct zones are formed. In this method it is important that the ratio between the quantities of antigen and antibody be proper.
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