Question

Importance of annealing temperature in real time pcr

Answer 1

PCR stands for polymerase chain reaction, and it's a laboratory procedure that can be used to create copies of DNA. The first step in a PCR cycle is the denaturation step. This is the PCR step in which the hydrogen bonds holding the complementary strands of DNA together are broken
If the annealing temperature is too high, primers are unable to bind to the template. ... The annealing temperature should not exceed the extension temperature. Denaturation temperature was too low. If the denaturation temperature is too low, the DNA will not completely denature and amplification efficiency will be low.
if it is high just the opposite will be done




hope you get it