Question

Importance of fixation and staining in biological science

Answer 1

Fixation. It is important that the original structure of the tissue is preserved before it reaches the histopathology laboratory. ... Cells and soft tissues tend to be more susceptible to breakdown than bone, cartilage, tendons and proteins of the extracellular matrix.

The Gram stain is the most important staining procedure in microbiology. It is used to differentiate between gram positive organisms and gram negative organisms. Hence, it is a differential stain. Gram negative and gram positive organisms are distinguished from each other by differences in their cell walls.