In a bacterial culture some of the colonies produced blue colour in the presence of a chromogenic substrate and others did not due to the presence or the absence of insert (rDNA) in the coding sequence of b-galactosidase.
(a) Mention the mechanism and the steps involved in the above experiment.
(b) How is it advantageous over simultaneous plating on two different plates having different
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The presence of chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert . ... Non-recombinant bacteria form blue colonies due to the activation of the enzyme alpha galactosidase which hydrolyses alpha glyctosyl moiety from glycolipid and glycoproteins.
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