Question

In a Gram stain, one step could be omitted and still allow differentiation between gram-positive and gram-negative cells. What is that one step?​

Answer 1

Answer:

In last step we generally use safranin , if this step omitted still we can differentiate between gram positive and gram negative.

Answer 2

Answer:

Omitting the last step Counter- Staining with Safranin will still allow differentiation.

Explanation:

Bacteria are generally divided into two types according to their property of staining- Gram-Positive and Gram-Negative. It is a differential staining method.

In Gram staining, Bacteria are stained with a primary stain like crystal violet, and a mordant like Iodine is poured to fix the stain, the bacteria which are able to retain the stain are Gram-positive whereas those that are decolorized by alcohol and are not able to take the stain are Gram-negative.

In my opinion, The final step in which we counter-stain bacteria with "Safranin" can be omitted as the Gram-positive will retain the purple color and the Gram-Negative will remain colorless.

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