Question

In a PCR, the concentration of each primer needs to be 10 micromolars. If the primer is 20 nucleotide long and Avg Mw of each nucleotide is 333 Da, What would be the concentration in microgram/ml ?​

Answer 1

Answer:

The nmol yield can be used to calculate concentration for your oligo. To get a standard 100uM concentration, you must add the nmol*10 volumen (uL). For instance, if your oligo was synthesized and the nmol yield is 44.2, then you must add 442uL of nuclease-free water to get 100 uM concentration.

Explanation:

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