Question

In an enzyme assay, the corrected absorbance reading obtained on addition of 100 l of the enzyme extract is 0.025. Given that one unit of the enzyme is the amount of enzyme required to increase the absorbance by 0.001 units under assay conditions, the enzyme activity (units/ml) of the extract is:

Answer 1

Answer:

Answer 250

Explanation:

This question is very logical, we do not need a formula to solve it.

0.001 absorption is obtained if we have 1 unit of enzyme. But absorption here is 0.025 so basically we need 25 units of enzyme to do this work.

We have 100 microlitre enzyme, and now we can say in this 100microlitre, 25 units are present.

But answer should be in units/ml

We got 25 units/100microlitre

So it will become 250 units/1ml.