Question

In an experiment to prepare temporary mount of a leaf peel,the stained leaf peel is put on a drop of glycerin. Explain why?

Answer 1

The leaf peel is stained using safranin solution to highlight the organelles. Glycerin prevents the leaf peel from drying. It also helps in refracting light as it is refractive medium. This helps in viewing the peel clearly under the microscope.

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Answer 2

Answer:

Glycerin is a dehydrating agent, which is used to avoid the drying of specimens of the leaf.

When glycerin is poured, it forms a protective layer that keeps it moisturized. That is why the peel is first stained, and then glycerin is put over.

If staining is done after adding glycerin, then the specimen will not stain properly, because it will be washed off with glycerin.