Question

In an experiment to prepare the temporary mount of a leaf peel to show stomata why glycerine and safranin are used ?

Answer 1

In experiment to prepare the temporary mount of a leaf peel to show stomata..we use glycerine to moist the leaf so that it guard cell can't be closed..or we use safranin because it is a stain which help us see clearly the cell in the stomata...
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Answer 2

The experiment to prepare the temporary mount of a leaf peel to show stomata uses safranin is used to peel off the upper layer of the stomata and also to stain it distinctly.

This highlights the cells while visualising under a microscope whereas the glycerine is used to keep the stomata wet in order to facilitate staining and viewing in the microscope, this works as a moisturiser and also protects the internal parts of the leaves.