in biotechnology which two enzymes are commonly called molecular scissor and Moleculer glue
Answers
Answer:
Restriction enzymes are also called 'molecular scissors' as they cleave DNA at or near specific recognition sequences known as restriction sites. These enzymes make one incision on each of the two strands of DNA and are also called restriction endonucleases.
Explanation:
The term scissors is used in molecular biology for an enzyme which is known as DNA restriction enzyme. The term glue is used in molecular biology for an enzyme which is known as DNA ligase. These enzymes are used for cutting and sticking the DNA during recombinant DNA technology.
Answer
Restriction enzymes are also called 'molecular scissors' as they cleave DNA at or near specific recognition sequences known as restriction sites. These enzymes make one incision on each of the two strands of DNA and are also called restriction endonucleases.
The term scissors is used in molecular biology for an enzyme which is known as DNA restriction enzyme. The term glue is used in molecular biology for an enzyme which is known as DNA ligase. These enzymes are used for cutting and sticking the DNA during recombinant DNA technology.
Molecular scissors are enzymes which cut the restriction ends of DNA. They cut the DNA at very specific places. The enzyme gets active only when it reads a particular sequence on the DNA molecule.
Restriction endonucleases are called molecular scissors as they cut the DNA segments at particular locations. Example: EcoRI.
DNA ligase
You can think of restriction enzymes as molecular scissors. DNA ligase is an enzyme that can join two DNA molecules. You can think of DNA ligase as molecular glue. DNA ligase joins two DNA molecules together by forming a phosphodiester bond between the two molecules.
DNA ligase in E. coli, as well as most prokaryotes, uses energy gained by cleaving nicotinamide adenine dinucleotide (NAD) to create the phosphodiester bond. It does not ligate blunt-ended DNA except under conditions of molecular crowding with polyethylene glycol, and cannot join RNA to DNA efficiently.
Explanation: