In callus culture roots can be induced by supplementing the nutrient medium with
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The IAA continues to accumulate in the callus. The concentration rises to the point that roots are initiated on the callus. The roots produce cytokinins (CK). ... As the concentration of cytokinin accumulates in the callus, it stimulates shoot formation.
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In callus culture, roots can be induced by the supply of higher concentration of auxin and lower concentration of cytokinin.
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