Question

in cell fractionation, the purpose of keeping the tissue sample in an isotonic solution in a refrigerator prior to homogenisation is a, to prevent osmotic damage to the cells and to reduce the metabolic activity of the cells b,to prevent osmotic damage to the cells and to increase the metabolic activity of the cells c,to prevent osmotic damage to the organelles and to reduce the metabolic activity of the cells d,to prevent osmotic damage to the organelles and to increase the metabolic activity of the cells​

Answer 1

Answer:

Tissue is typically homogenized in a buffer solution that is isotonic to stop osmotic damage. Mechanisms for homogenization include grinding, mincing, chopping, pressure changes, osmotic shock, freeze-thawing, and ultra-sound. The samples are then kept cold to prevent enzymatic damage.

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