In confocal scanning laser microscope, the image is built up pixel by pixel by collecting the emitted Photon from which compound that acts like a dye present in the sample?
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The CLSM works by passing a laser beam through a light source aperture which is then focused by an objective lens into a small area on the surface of the sample and an image is built up pixel-by-pixel by collecting the emitted photons from the fluorophores in the sample.
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